Diagnosis value of expression of ICAM-1 and LFA-1 in acute rejection of liver transplantation in rats
- VernacularTitle:肝组织ICAM-1及LFA-1表达对急性排斥反应的诊断价值
- Author:
Yuyang FU
;
Xiaoshun HE
;
Jianlin CHEN
;
Guihua CHEN
;
Jiefu HUANG
- Publication Type:Journal Article
- Keywords:
LIVER TRANSPLANTATION/immunol;
GRAFT REJECTION;
LIVER/pathol;
INTERCELLULAR ADHESION MOLECULE;
LYMPHOCTIE FUNCTION ASSOCIATED ANTIGENS
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the intercellular adhesion molecule-1(ICAM-1) and lymphocyte function associated with antigen-1( LFA-1) and acute rejection of liver transplantation in rats. Methods SD rats and Wistar rats were randomly divided into normal control group, isograft group, and allograft group. Liver biopsies from the recipients were obtained on the day 2,4, and 7 postoperatively to examine the expression level of ICAM-1 and LFA-1 in liver grafts. Samples were firstly stained with specific monoclonal antibodies using immunohistochemistry stain and then applied to image semiquantitive analysis. Results The expression levels of ICAM-1 and LFA-1 in allograft group were obviously higher than those in both isograft group and normal group. Moreover, the expression levels of ICAM-1 and LFA-1 were correlated positively with the degree of acute liver rejection. Conclusions Detecting the expressions of ICAM-1 and LFA-1 in liver graft tissue may be helpful to the diagnosis of acute rejection after liver transplantation.
