Effects of LPS on expressions of ET-1, eNOS and iNOS mRNA in human umbilical vein endothelial cells
- VernacularTitle:脂多糖对人脐静脉内皮细胞ET-1、eNOS、iNOS mRNA表达的影响
- Author:
Peng LI
;
Shanshan LI
;
Qifu HUANG
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Umbilical veins;
Endothelium;
Endothelin-1;
Nitric oxide synthase
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the direct effect of LPS on expressions of ET-1, eNOS, and iNOS mRNA in human umbilical vein endothelial cells, and further research the molecular mechanism of effect of LPS on production of ET-1 and NO. METHODS: The third passage of cultured human umbilical vein endothelial cells was incubated with low concentration (100 ?g/L) of LPS for 6 hours. Total RNA was extracted. The expressions of ET-1, eNOS, and iNOS mRNA were analyzed by semi-quantitative RT-PCR method. RESULTS: ET-1 mRNA experession increased significantly, while expression of eNOS mRNA decreased significantly, and there was no significant change in expression of iNOS mRNA. CONCLUSIONS: In human umbilical vein endothelial cells, low concentration of LPS enhanced the expression of ET-1 mRNA, inhibited the expression of eNOS mRNA, and had no significant effect on the expression of iNOS mRNA.
