Change of phosphoinositide signaling pathway in vascular endothelial cells exposed to projectile's pressure wave
- VernacularTitle:高速投射物压力波作用下内皮细胞磷酸肌醇信号通路的变化
- Author:
Lili WANG
;
Xinan LAI
;
Yinchu SHAO
- Publication Type:Journal Article
- Keywords:
Pressure;
Endothelium, vascular;
Signal transduction
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the phosphatidylinositol metabolism of vascular endothelial cells exposed to projectile's pressure wave METHODS: The cultured endothelial cells were exposed to bullet's pressure wave as experimental model. The intracellular inositol-1,4,5-triphosphate(IP_3), intracellular free calcium ([Ca 2+ ]i), protein kinase C(PKC) and lactate dehydrogenase (LDH) activities in cultural medium were assayed after the cultured cells exposed to bullet's pressure wave. After blocking phosphatidylinositol metabolism with neomycin, the above biochemical parameters of cells exposed to bullet's pressure wave were also measured. RESULTS: Bullet's pressure wave induced increase in the intracellular IP_3, [Ca 2+ ]i, and PKC activity obviously. The change of LDH activity paralleled with above biochemical changes. Blocking phosphatidylinositol metabolism partially inhibited these changes. CONCLUSION: Bullet's pressure wave may activate phosphatidylinositol metabolism, which may trigger the injury to the endothelial cells and lead to leakage of plasmatic LDH.
