Protein expression of cyclin D_2 and p16 in proliferation and differentiation of cultured cardiac myocytes
- VernacularTitle:Cyclin D_2和p16蛋白表达在心肌细胞增殖、分化中的变化
- Author:
Yuxia ZHANG
- Publication Type:Journal Article
- Keywords:
Cyclins;
Protein p16;
Myocardium;
Cell cycle
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
AIM: To investigate the protein expression of cyclin D_2 and p16 in proliferation and differentiation of cultured cardiac myocytes.METHODS: One-day-old Sparague-Dawley rats were used. Cardiac myocytes(CM) were collected by a trypsin-dispersal method and cultured. Cell growth line and fluorescence activated cell sorting (FACS) were used to investigate the proliferation of CM. Ultra-thin sections were made to observe the ultrastructure of CM under transmission electron microscope. The expression of cyclin D_2 and p16 in CM were measured using immunocytochemistry and image analysis.RESULTS: ①Results of cell growth line and FACS analysis showed that cultured CM could proliferate in the first 3 cultured days, but the ability decreased quickly, concomitant with differentiation. CM was obseved quiescent in cell cycle three days later. The ultrastructure of CM showed the large amount of myofilaments and mitochondrion. ②The protein expression of cyclin D_2 in 3,4,5 day CM group was 0.89 times( P
