Effect of SC58125 on cell proliferation and apoptosis in HepG-2 cells
- VernacularTitle:SC58125对HepG-2细胞增殖和凋亡的影响
- Author:
Xiaolong KE
;
Hongliang LI
;
Dandan CHEN
;
Xianda REN
;
Haiwei ZHANG
;
Xiaodong CHEN
;
Aihu XIONG
- Publication Type:Journal Article
- Keywords:
Anti-inflammatory agents, non-steroidal;
Apoptosis;
Liver neoplasms;
SC58125
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To clarify the effect of SC58125 on cell proliferation and apoptosis in HepG-2 cells and explore the molecular mechanisms. METHODS: Cell culture, MTT, TUNEL, DNA ladder, flow cytometry and Western blot analysis were employed in the present study. RESULTS: SC58125 inhibited the growth of HepG-2 cells and induced the apoptosis. Furthermore, it arrested G_0/G_1 phase and inhibited S phase in HepG-2 cells. Depressed expression of P33 cdk2 ,P34 cdc2 ,cyclin B_1,cyclin E ,Mpm-2,Rb ,PCNA proteins were found in HepG-2 cells treated with SC58125. CONCLUSION: SC58125 inhibits cell proliferation and induces apoptosis, which may be related to the altered low protein levels of P33 cdk2 ,P34 cdc2 ,cyclin B_1,cyclin E ,Mpm-2,Rb,PCNA
