Effects of ischemic preconditioning on hepatocyte apoptosis induced by hepatic ischemia reperfusion in cirrhotic rats
- VernacularTitle:缺血预处理对肝硬化大鼠肝脏缺血再灌注中肝细胞凋亡的影响
- Author:
Shaoqiang LI
;
Lijian LIANG
;
Jiefu HUANG
- Publication Type:Journal Article
- Keywords:
Ischemia;
Liver cirrhosis;
Apoptosis;
Reperfusion injury;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the protective effect of ischemic preconditioning (IPC) on hepatic ischemia reperfusion(I/R) injury in cirrhotic rats and its possible mechanism. METHODS: Hepatic I/R was induced by Pringle maneuver. The cirrhotic rats were randomized into three groups: Group A: before 30 min of ischemia, a short period of 5 min ischemia and 5 min reperfusion were given; Group B: before 30 min of ischemia, a short period of 10 min ischemia and 10 min of reperfusion were given; Group C: 30 min ischemia only. The serum alanine transferase (ALT), hepatic Fas mRNA, caspase 3 activity and hepatocyte apoptosis were analyzed. RESULTS: The 7 day survival rate in the group A and B were 100%, respectively. However, it was only 62.5% in the group C. After 6 h of reperfusion, the ALT levels in both group A and B were significantly lower than that of in group C, P
