Involvement of p38 kinase signaling in PC12 cell death induced by nitric oxide
- VernacularTitle:p38应急信号通路参与一氧化氮诱导PC12细胞死亡
- Author:
Chengyi LUO
;
Ruxiang XU
;
Zhilin YANG
;
Zhiliang LIU
- Publication Type:Journal Article
- Keywords:
Nitric oxide;
PC12 cells;
Cell death;
Signal transduction
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study signal transduction pathway of nitric oxide-induced PC12 cell death. METHODS: After PC12 cells were incubated with sodium nitroprusside(SNP), caspase-3 inhibitor Ⅱ plus SNP or p38 inhibitor-SB203580 plus SNP, cell survival rate was quantified by MTT assay and caspase-3 activity was measured with caspase-3 assay kits. RESULTS: SNP induced PC12 cell death in a dose- and time-dependent manner, and increased caspase-3 activity gradually. Both caspase-3 inhibitorⅡand SB203580 reduced cell death significantly, but SB203580 reduced caspase-3 activity significantly. CONCLUSION: NO might induce PC12 cell death through the activation of p38 and caspase-3.
