Down-regulation of ?-defensin-2 gene expression in E.coli-infected rat lung tissue
- VernacularTitle:大肠杆菌感染对大鼠肺组织?-防御素-2基因表达的负相调节作用
- Author:
Xinnian CHEN
;
Ning HUANG
;
Qi WU
;
Boyao WANG
- Publication Type:Journal Article
- Keywords:
Rats;
Lung;
Gene expression;
Enterobacteriaceae
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the regulation of rat ?-defensin-2 gene expression by bacteria.METHODS: E.coli ML-35 p and pseudomonas aeruginosa were injected into rat trachea. Total RNA was isolated from lung tissue after 24 h inoculation. RT-PCR and Northern blot were performed to detect ?-defensin-2 ( rBD-2 ) mRNA expression.RESULTS: The expression of rBD-2 gene was inhibited in the lung tissue by E.coli infection, but not by pseudomonas aeruginosa. CONCLUSION: These results indicated that E.coli infection could down-regulate rBD-2 gene expression in the rat lung tissues.
