The study of enriching peripheral circulating carcinoma cells in colorectal cancer patients by magnetic activated cell sorting
- VernacularTitle:磁激活细胞分选技术用于结直肠癌外周血癌细胞富集的研究
- Author:
Zhihai PENG
;
Fang ZHANG
;
Yun LING
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Neoplasm circulating cells;
Flow cytometry
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo enrich and quantitatively analyze peripheral circulative carcinoma cells by magnetic activated cell sorting (MACS) in colorectal cancer patients.MethodsBlood samples, preoperatively collected from 21 patients with colorectal cancer and 9 healthy volunteers, were labeled by anti-cytokeratin (CK) with magnetic microbeads, and passed through magnetic columns; CK +carcinoma cells were separated from the samples, and quantified by flow cytometry.ResultsCK +CD45 -cells could not be detected from the samples without MACS; in the sample undergoing MACS, the concentrations of CK +CD45 -cells were significantly higher in patient group than in control group(P
