Determination of the Concentration of Valproic Acid in Serum With RP-HPLC
- VernacularTitle:反相高效液相色谱法测定人血清中丙戊酸钠浓度
- Author:
Lin HE
;
Jiying YU
;
Zhengzhong WU
;
Shiming SUN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
valproic acid;
derivation
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the concentration of valproic acid in serum.METHODS:Determination was performed with RP-HPLC with methanol:water(70∶30) as mobile phase,?-bromoacetophenone as deriving agent and cyclohexanecarboxylic acid as internal standard,and detected at wavelength 248nm.RESULTS:The calibrating curve of valproic acid was linear in the range of 14.47～248.0?g/ml.CONCLUSION:The method was convenient,rapid,accurate and suitable for TDM.
