Determination of the Enantiomers of Ceftriaxone Sodium by Capillary Electrophoresis
- VernacularTitle:毛细管电泳测定头孢曲松钠对映体含量
- Author:
Jingwen XIE
;
Rong WANG
;
Zhengping JIA
;
Xiaoli HU
;
Hua XIE
- Publication Type:Journal Article
- Keywords:
capillary electrophoresis;
enantiomeric separation;
?-cyciodextrin;
ceftriaxone
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a capillary electrophoresis method for determing the enantiomer of ceftriaxone Na.METHODS:A chiral resolving agent,?-cyclodextrin,was employed as chiral additive for ceftriaxone Na enantiomeric separation by capillary electrophoresis.In different electrophoresis polarity mode,the effect of pH of background electrolyte and the concentration of ?-cyclodextrin were investigated.RESULTS:The optimal conditions for enantiomeric separation were as follows:separation voltage:28kV,buffer solution:NaH2 PO4 50mmol/L,?-CD 0.04mmol/L,Tirs 3.0mmol/L,pH7.15.CONC_LUSION:The method is simple,sensitive,rapid and accurate,and can be used for the quality control of ceftriaxone Na enantiomers.There was significant difference in contents of enantiomers of ceftriaxone Na between products of two factories.We suggest that a quality control method for ceftriaxone Na enantiomer should be established,which will provide scientific basis for quality control of drug and clinical choice of effective antibioties.
