Effects of three kinds of calcium activator on the proliferation of cultured vascular smooth muscle cells in rats
- VernacularTitle:3种钙激动剂促培养的大鼠血管平滑肌细胞增殖
- Author:
Yongjian YANG
;
Xin ZHANG
;
Xinwen ZHOU
;
Jun ZHU
;
Longsheng ZHAO
- Publication Type:Journal Article
- Keywords:
Mitogens;
Protein kinases;
Muscle, smooth, vascular;
Cells;
Calcium
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of intracellular free calcium ([Ca 2+ ]i) from different resources on the proliferation mediated by mitogen activated protein kinase (MAPK) in vascular smooth muscle cells (VSMCs). METHODS: Cultured VSMCs were used in all experiments. Calcium influx was stimulated by angiotension Ⅱ(AngⅡ). The release of intracellular calcium stores was induced by inositol trisphosphate(IP 3) and ryanodine(RY). MAPK activity was measured by [?- 32 P]-ATP incorporation , MAPK protein expression by western blot, VSMCs proliferation by -Leucine(-Leu) and -Thymidine(-TdR) incorporation. RESULTS: Compared to the control VSMCs, AngⅡ, IP 3 and RY significantly increased [Ca 2+ ]i concentration , activity of MAPK and its protein content in VSMCs. The promotion of -Leu and -TdR incorporation in VSMCs was also observed ( P
