Alterations of cardiac sympathetic norepinephrine transporter expression in rats with volume overload
- VernacularTitle:长期容量超负荷心力衰竭大鼠心脏交感神经去甲肾上腺素转运蛋白表达的变化
- Author:
He LI
;
Xiaoping HU
;
Sunkai MA
;
Jian FEI
;
Guoyuan ZHANG
;
Zonggu WU
- Publication Type:Journal Article
- Keywords:
Norepinephrine;
Receptors, adrenergic, beta;
Gene expression;
Carrier proteins
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the alterations of myocardial ? 1-adrenergic receptor (? 1-AR) and cardiac sympathetic norepinephrine transporter (NET) mRNA expression, which is upstream modulator of ? 1-AR, in rats with longterm volume overload (VOL).METHODS: Left ventricular systolic (LVSP) and end diastolic pressure (LVEDP) of rats with VOL induced by aortacaval fistula operation and control group were measured at 3, 14,30 and 60 d after the operation, the mRNA at the time points was measured by RT-PCR and Northern blot analysis and quantified by densitometry.RESULTS: The cardiac sympathetic NET specific expression is in the cardiac sympathetic ganglia. Be compared with the control group, LVSP of VOL rats decreased most dramatically by 24%( P
