Roles of NF-?B, AP-1 and caspase-3 in SC58125 induced apoptosis in HepG2 cells
- VernacularTitle:NF-?B,AP-1及caspase-3在SC58125诱导的HepG2细胞凋亡中的作用
- Author:
Xiaolong KE
;
Hongliang LI
;
Dandan CHEN
;
Xianda REN
;
Haiwei ZHANG
;
Xiaodong CHEN
;
Jianju LIU
- Publication Type:Journal Article
- Keywords:
Anti-inflammatory agents, non-steroidal;
Apoptosis;
NF-kappa B;
Liver neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the molecular mechanisms of SC58125 on apoptosis in HepG2 cells. METHODS: Cell culture, ELISA, flow cytometry, RT-PCR, Western blot and electrophoretic mobility shift assay (EMSA) analysis were employed to clarify the effect of SC58125 on apoptosis in HepG2 cells and related molecular mechanisms. RESULTS: SC58125 induced apoptosis in HepG2 cells in a concentration-dependent manner, which was accompanied by inhibition of NF-?B, activation of caspase-3, decrease of bcl-2 mRNA and increase of p53 mRNA. However, no significant changes were found in the DNA binding of AP-1. CONCLUSION: SC58125 induces apoptosis in HepG2 cells, which may be related to the inhibition of NF-?B, activation of caspase-3, decrease of bcl-2 mRNA and increase of p53 mRNA.
