Effect on the expression of proto-onogene c-mpl in megakaryocytes line-HEL by interleukin-13
- VernacularTitle:白细胞介素-13对巨核细胞系-HEL细胞原癌基因c-mpl表达的影响
- Author:
Mingyan XU
;
Xiaoyu SHI
;
Wenli LI
- Publication Type:Journal Article
- Keywords:
Interleukin-13;
Megakaryocytes;
Cell line;
Proto-oncogenes;
Gene expression
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the relevance of the proliferation of megakaryocytic cell line-HEL stimulated by the recombinant human interleukin-13 (IL-13) to the expression of pro-oncogene c-mpl in HEL cells. METHODS: MTT colorimetric assay and reverse transcrition polymerase chain reaction (RT-PCR) are separately used in this study to observe the effect on the proliferation of HEL cells and the expression of c-mpl mRNA in HEL cells by rhIL-13. RESULTS: RhIL-13 stimulated the proliferation of HEL cells and upregulated the expression of c-mpl mRNA in HEL cells. CONCLUSION: Our results suggest that rhIL-13 stimulated the proliferation of HEL cells and provide the evidence that its mechanism is partly because of increasing the pro-oncogene c-mpl expression in HEL cells.
