Changes in platelet-activating factor receptor binding characteristics in cerebral thrombotic core and penumbra of tree shrews
- VernacularTitle:树血栓性脑缺血中心区及半暗区神经元PAF受体结合特性的研究
- Author:
Lijun YANG
;
Shuqing LI
- Publication Type:Journal Article
- Keywords:
Photochemistry;
Cerebral ischemia;
Platelet activating factor;
Tupaiidae
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the changes in platelet-activating factor(PAF) receptor binding characteristics and explore the action of PAF on formation of thrombotic core and penumbra following local cerebral ischemia.METHODS: Neuron's membrane protein was abstracted, and the local cerebral ischemia model were induced by photochemistry in tree shrews. The PAF binding sites on central neuron membrane were studied by -PAF binding assay.RESULTS: There were two different affinities of PAF receptors on tree shrew's brain cell membrane, with kD 1=( 3.61 ? 0.72 ) nmol/L and kD 2=(17 04?2 41) nmol/L, corresponding respectively to maximum number of binding sites: Bmax1=(1 457 94?168 01) pmol/g protein and Bmax2=(5 017 40?742 16) pmol/g protein. The binding sites decreased in ischemic core, penumbra and contralateral regions at 4,24 and 72 h after ischemia ( P
