Functional study of hepatocytes cultured in sandwich configuration
- VernacularTitle:体外培养条件下肝细胞功能研究
- Author:
Xianjie ZHANG
;
Jiabang SUN
;
Duo LI
- Publication Type:Journal Article
- Keywords:
Cells culture;
Liver function tests;
In situ hybridization
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new culture system in which hepatocytes can restore in-vivo like function. Methods Sandwich configuration was used for the culture of hepatocytes, and protein synthesis as well as P 450-b ,albumin mRNA expression by in situ hybridization was studied. ResultsStable and gradually increasing protein synthesis and high level of P 450-b , albumin mRNA expression were observed. In contrast, protein secretion of hepatocytes cultured in conventional single collagen culture system was low and decreased until approximately zero seven days after, and mRNA expression of P 450-b ,albumin was low. Conclusions Sandwich configuration in which cell polarity of hepatocytes is reestablished like in vivo situation.
