Therapeutic potential of recombinant adenovirus expressing p53 on hepatocellular carcinoma cells possessing different p53 functional status
- VernacularTitle:野生型p53基因对不同内源性p53状态的肝癌细胞株生长的影响
- Author:
Ying GUO
;
Kun WANG
;
Jisheng CHEN
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Genes, p53;
Gene expression regulation, neoplastic;
Adenovirus
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of exogenous wild-type p53(WT-p53) on the growth of hepatocellular carcinoma (HCC) cell lines possessing different p53 functional status.Methods With recombinant adenoviral vector expressing WT-p53(Ad-p53),p53 gene was transfected into HCC cell lines:Bel-7402,QGY-7701, and PLC/PRF/5.The cytotoxicities of Ad-p53 to these cell lines were measured by MTT assay,cell growth properties and cell cycle pattern were assessed by flow cytometry.Results The OD of PLC/PRF/5, Bel-7402,and QGY-7701 cells transfected with Ad-p53 of 40,200,and 800 MOI were 0.02?0.00 vs 0.98?0.16(P0.05),compared with control groups respectively. Analysis of flow cytometry showed higher G2/M? lower S and apoptosis ratio in PLC/PRF/5 cells,while higher G0/G1 ratio and lower S ratio in Bel-7402 cells. ConclusionReplication-deficient adenoviral vector expressing WT-p53 may be useful for gene therapy in HCC regardless the status of endogenous p53.
