An experimental study on the gene and protein expression of TGF ?_1 and its relationship with the wound age during the healing process of incised wound of rat's skin
- VernacularTitle:在大鼠皮肤切创愈合过程中TGF-?_1基因及蛋白表达变化与损伤时间关系的实验研究
- Author:
Huijun WANG
;
Dong LI
;
Mulan YANG
- Publication Type:Journal Article
- Keywords:
Forensic pathology;
Expression of TGF ? 1;
Timing of incised skin wound
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the relationship between the expression of TGF ? 1,its receptor and the wound age during the healing process of rats skin incision wound,a preliminary study were performed on the vital skin wound 0 5～168h after injury by using the immunohistochemical and the molecular biological methods.The results were then compared with those of the postmortem skin injury.The results revealed that the expression of the cytokine TGF ? 1 in the epithelial cells were enhanced 0 5h after the antemortem skin incision and the strongest reactions were seen at 24～96h post injury.The expression of TGF ? 1 was also found in the macrophages and the fibroblasts in the granulation tissues.Analysis of the immunoblotting(Western blot)results showed that the peak value of the TGF ? 1 protein was appeared at 168h after the antemortem skin incision and peaked at 96h.TGF ? 1 was detected 0 5～3h after postmorten skin injury by using the immunohistochemical method.No expression of mRNA was found.It is suggested that some regular and characteristic expression of TGF ? 1 in the incised skin wounds were related with the wound age.It might be used for timing of skin wound on rat.
