The relationship between electrophysiological and morphological properties of neurons in visual cortex of developing rat-intracellular staining study
- VernacularTitle:大鼠发育过程中视皮层神经元电生理与形态学特性的相关性——细胞内染色研究
- Author:
Yali TU
;
Zhengqin YIN
;
Yingbing LIU
- Publication Type:Journal Article
- Keywords:
Optic Nerve/physiology;
Neurons/physiology;
Electrophysiology;
Patch-clamp techniques;
Biotin;
Animal testing alteruatives
- From:
Chinese Journal of Ocular Fundus Diseases
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between electrophysiological and morphological properties of neurons in visual cortex of developing rat, speculate the coincided degree between electrophysiological and morphological change and realize the mechanism of normal visual development. Methods Whole cell patch-clamp recording and intracellular staining were used to acquire cellular microelectrode recording in visual cortex from Sprague-Dawley rats (4~28 days old). The histological process was made. Results The differences of electrical feature between pyramidal cells and non-pyramidal cells were significant. The morphological maturity degree is different in developing visual cortex. Conclusion The different function of pyramidal and non- pyramidal cells in local integrition is reflected by their electrical feature in the process of visual development. In critical period of visual development, the coincision degree of the electrophysiological and morphological change in visual cortex is larger than that in the subcortex constructure.
