Effects of aspirin on production of nitric oxide and inducible nitric oxide synthase mRNA expression under inflammatory conditions in human vascular endothelial cells
- VernacularTitle:阿司匹林对炎症条件下人血管内皮细胞一氧化氮的产生及诱导型一氧化氮合酶mRNA表达的影响
- Author:
Zhixu HE
;
Qinkui LIAO
;
Xueju XU
;
Chunhua LUO
;
Tongfu ZHOU
;
Qinbo LI
;
Fengyi LI
;
Shure WANG
- Publication Type:Journal Article
- Keywords:
Aspirin;
Nitric oxide;
Inflammation;
Endothelium;
Cells;
Nitric oxide synthase
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of aspirin on inducible nitric oxide synthesis and gene expression under inflammation in endothelial cells. METHODS: Using NADPH, Griess methods and RT-PCR, the activity of isozymes of NO synthase (NOS), nitric oxide (NO) level, and iNOS mRNA expression were examined respectively. Also, the lactate dehydrogenase (LDH) release rate, malondialdehyde (MDA) content and cell viability were measured. RESULTS: Aspirin (3 mmol/L) reduced inducible NO production and NOS activity(P
