Experimental Study on the Effects of Panax Notoginsengnoside on Neutrophil Functions in Rats With Air-vesicle Synovitis and Its Mechanisms
- VernacularTitle:三七叶皂苷对气囊滑膜炎大鼠中性粒细胞功能的影响及其机制研究
- Author:
Xiaohui LI
;
Shuhui LI
- Publication Type:Journal Article
- Keywords:
Panax notoginsengnoside;
neutrophil;
phagocytosis;
O 2 ?-;
Ca2+
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study of effects of Panax notoginsengnoside(PnGL) on neutrophil(Neu) functions in carrageenin(Car) induced subcutaneous air-vesicle synovitis in rats and its mechanisms METHODS:Rat air-vesicle synovitis model was established with sc Car(25mg/kg) Neu count,the phagocytosis of Neu,the realease of O2 ?- from Neu and the level of Neu-i were measured with test tube diluting method,staphylococcus aureus phagocytosis method,cytochrome C reduction method and Fura-2 fluorescence technique respectively RESULTS:At 12h after Car used,Neu count of exudates elevated,phagocytosis rate and index of Neu and the release of O2 ?- from Neu increased obviously,suggesting that Neu was in an activated state Mea_nwhile,the level of Neu-i elevated significantly PnGL 60～240mg/kg ip,30min before and 6h after Car sc,reduced Neu count of exudates,inhibited phagocytosis functions and the increase of i level of Neu in a dose-dependent manner There was a significant correlation between the reduction of Neu-i and the inhibition of Neu phagocytosis functions and the release of O2 ?- from Neu Dexamethason had similar effects on Neu functions to PnGL,but with no effect on the level of Neu-i CONCLUSION:The reduction of Neu-i level and the subsequent inhibition of Neu functions is one of the important anti-inflammatory mechanisms of PnGL,which is different from that of Dex
