Th1 Cytokine ( IFN-gamma ) Secretion Pattern of Peripheral Blood Mononuclear Cells Response to Trophoblast Antigen in Women with Unexplained Recurrent Spontaneous Abortion and Normal Fertile Controls.
- Author:
Keun Jai YOO
;
In Ok SONG
;
Bum Chae CHOI
;
Inn Soo KANG
;
In Sou PARK
;
Hye Kyung BYUN
;
Ji Ae LEE
;
Jeong Wook KIM
;
Hyun Joo KIM
- Publication Type:Original Article
- Keywords:
Recurrent spontaneous abortion;
Trophoblast antigen;
Thl-cytokine (IFN-r )
- MeSH:
Abortion, Spontaneous*;
Cell Line;
Cytokines;
Enzyme-Linked Immunosorbent Assay;
Female;
Humans;
Hypersensitivity;
Pregnancy;
Reproductive History;
Trophoblasts*
- From:Korean Journal of Obstetrics and Gynecology
1998;41(12):3063-3068
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: A dichotomous Thl and Th2 cytokine profile has been associated with reproductive failure and success, respectively. The purpose of our study was to determine the levels of Thl cytokine (IFN- y ) secreted by peripheral blood mononuclear cells (PBMCs) form women with unexplained recurrentabortion (URA) and fertile controls in response to trophoblast antigen. METHODS: PBMCs were isolated from 30 nonpregnant women with URA and from 10 nonpregnant fertile controls. Following 4 days of culture (1 * 10(6) cells/mL) with and without a protein extract derived from a trophoblast cell line (30 ug/mL, protein). None of the women had allergies, atopy or recent infection. Cytokines were measured in supernatants with enzyme-linked immunosorbent assay (ELISA) kits. IFN- r kit was obtained from BOISOURCE (lower limit of sensitivity, 15.6 pg/mL for IFN- r ). All values below the lowest limit of sensitivity as determined by test kit standards were considered negative. The cytokine stimulation test is considered positive if the IFN- r concentration increases by 200% or more with the trophoblast antigen stimulation. Datas are presented as mean+ SEM. Nonparametric testing (Mann-Whitney U) was used for analysis with P<0.05 considered statistically significant. RESULTS: The Thl-type cytokine (IFN- r ) was detected in 20(67%) of 30 supernatants from women with URA. In contrast, 2 (20%) of trophoblast-activated PBMC culture supernatants from the 10 parus women with normal reproductive histories was detected IFN- r and but were significantly lower than levels in women with URA who had secreted IFN- r upon trophoblast stimulation (99.80+ 18.17 pg/mL versus 166.47 + 36.96 pg/mL, p<0.05). Spontaneous secretion of IFN- r was significantly higher in culture supernatants from women with URA than in supernatants from women with successful reproductive histories (41.36.09+6.99 pg/mL versus 25.89+9.34 pg/mL, p<0.05). CONCLUSION: These data indicate that there are significant differences between women with URA and women with normal reproductive histories in their regulation of the Thl-cytokine (IFN- r) in response to trophoblast. Thl-type immunity to trophoblast is associated with URA and may play a role in reproductive failure.
