Effect of lidocaine on Bcl-2 and CPP32 protein expressions of hippocampus neurons after transient global cerebral ischemia
- VernacularTitle:利多卡因对短暂脑缺血后海马区细胞Bcl-2和CPP32蛋白表达的影响
- Author:
Liping LIU
;
Xinmin WU
;
Yuan LIU
- Publication Type:Journal Article
- Keywords:
Genes,bcl 2;
Caspases;
Cerebral ischmia, transient;
Lidocaine
- From:
Chinese Journal of Anesthesiology
1997;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of lidocaine on B cell lymphoma 2 gene (Bcl 2) and CPP32 protein expressions of hippocampus CA1 neurons after transient global cerebral ischemia Both genes are closely related to the development of apoptosis Methods Twenty four healthy adult rabbits weighing (3 0?0 2)kg were randomly divided into three groups: sham operative group (SH group, n=6); ischemia group (IS group, n=9) received normal saline 1 5ml I V 5min before ischemia and lidocaine group (LI group, n=9) received 2% lidocaine 10 ml/kg I V 5 min before ischemia Global cerebral ischemia was induced by the occlusions of bilateral common carotid and vertebral arteries All the rabbits were decapitated 24 h after ischemia and brain tissue was removed immediately Expressions of Bcl 2 and CPP32 proteins of hippocampus CA1 neurons were detected by immunoblotting Results Expression of Bcl 2 in IS group and LI group increased significantly as compared with that in SH group (P
