Role of heme oxygenase-1 in the ischemic preconditioning of isolated rat heart
- VernacularTitle:血红素氧化酶-1在离体大鼠心肌缺血预处理中的作用
- Author:
Jie HE
;
Min ZHANG
;
Junchang LIU
;
Liling WU
;
Yijin WU
- Publication Type:Journal Article
- Keywords:
Ischemia;
Myocardial reperfusion;
Heme oxygenase
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the influence of ischemic preconditioning on heart function, the activities of lactate dehydrogenase(LDH), malondialdehyde(MDA), and heme oxygenase-1(HO-1) after ischemia/reperfusion in isolated rat heart. METHODS: The model of Langendorff was used in isolated rat heart perfusion. Ischemic preconditioning protocol: stopping perfusion for 5 minutes and reperfusion for 5 minutes, repeating three times. Ischemia protocol: stopping perfusion for 40 minutes and reperfusion for 20 minutes. Indexes of heart function were recorded in control group, ischemia and reperfusion group(IR), and ischemic preconditioning group(IPC). The content of LDH of coronary effluent was measured. Moreover, the content of MDA and activity of HO-1 in myocardium were also measured. RESULTS: The recovery percentage of heart function in IPC group was significantly higher than that in IR group(P
