Immunohistochemical observation and imaging analysis of iNOS and vasoactive intestinal peptide in experimental diabetic rat lung
- VernacularTitle:实验性糖尿病肺iNOS和VIP免疫组织化学观察及图像分析
- Author:
Xingping SHEN
;
Changda SHU
;
Ju HE
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus, experimental;
Vasoactive intestinal peptide;
Nitric oxide
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The pathological changes in inducible nitric oxide synthase (iNOS) and vasoactive intestinal peptide (VIP) in diabetic rat lung were investigated.METHODS: Using immunohistochemical method and imaging analysis, the changes in iNOS and VIP were observed in normal and diabetic rat lung. RESULTS: In diabetic rats, the iNOS positive or weak positive staining was localized in epithelial cells of bronchi, epithelial cells of alveolar ducts, alveolar sacs, and arteries, but negative in endothelial cells of vein and capillary. Image analysis showed the area, the integral absorbance(IA) and relative contents of iNOS were significant lower than that of control (P
