Study on Antigenic Specificity of Candida albicans Enolase
- VernacularTitle:白念珠菌烯醇化酶的抗原特异性研究
- Author:
Ningfeng TANG
;
Ruiqing JIAO
;
Shaoxi WU
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Enolase;
Antigens
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the antigenic specificity of the enolase.Methods and Results The results included:(1)Western blot analysis demonstrated that enolase was a main protein in the nonglyco-proteins of C.albicans which could react with the serum of C.albicans infected rabbit.(2)The purified enolase was used to immunize rabbit for producing anti-enolase serum,and the hig hest titer of polyclonal antibody against enolase was up to 1:6400with enzyme-linked immunoadso rbent assay(ELISA).(3)Compared with three other polyclonal antibodies,anti-enola se antibody showed high specificity in detecting C.albicans antigen.Conclusion Enolase as a specific antigen,is worthy of further investigation in C.albicans cell.
