Interleukin-4in Experimental Murine Systemic Candidiasis
- VernacularTitle:小鼠白念珠菌系统性感染中白介素4表达的研究
- Author:
Jiawen LI
;
Daofan LIU
;
Jiqun CHENG
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Interleukin -4;
Candidiasis,murine
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of interle ukin -4(IL -4)in experimental murine systemic candidiasis.Methods In the dexamethasone -induced immuno -suppressed murine systemic can-didiasis models and control healthy mice,two -site ELISA and RT -PCR were applied to determine the levels of IL -4protein or mRNA expression in th e spleens respectively.Colony form ing units(CFUs )of infected kidneys were determined with the plating dilution method,and the mean survival t ime (MST)of mice was also recorded.Results The IL -4concentration in the spleen of healthy mice was 80.1?19.1pg /g.I n contrast,in those infected with lethal dose of yeasts t he concentrations of IL -4were 124.8?24.1pg /g and 262.8?21.8pg /g on 3days or 7days after infection respectively.Meanwhile,the fungal lo ads in kidneys were(21.25?6.31)?102CFUs and(57.52?10.41)?102 CFUs on 3days or 7days after infectio n(P
