Cloning and Expression of Histidine Kinase Gene of Aspergillus Fumigatus
- VernacularTitle:烟曲霉组氨酸激酶基因片段的隆及表达分析
- Author:
Chen DU
;
Ruoyu LI
;
Shengqing MA
- Publication Type:Journal Article
- Keywords:
Aspergillus fumigatus;
Histidine kinase;
Genes
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through isolating the histidine kin ase gene of Aspergillus fumigatus,to detect its role in the invasive aspergillosis.Methods Using degenerate primers for highly conserved regions of his-tidine kinase,RT -PCR was performed with cDNA of Aspergillus fumigatus as a template.The gene expression of it in vitro and in vivo was investig ated by Northern blot.Results A fragment of this gene was cloned fro m Aspergillus fumigatus that is highly homologous to tesA gene of Aspergillus nidulans,which was expressed at high level during invasive infection.Conclusion The results indicate that this gene may attribute to the invasive aspergillosis.
