Diagnosis of acute rejection by bile cytology and cytokine gene expression in rat liver transplantation model
- VernacularTitle:胆汁细胞学及细胞因子基因表达诊断大鼠肝移植急性排斥反应的实验研究
- Author:
Chi XU
;
Jiefu HUANG
;
Guihua CHEN
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Bile cytology;
Cytokine gene expression;
Acute rejection;
Diagnosis
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate a reliable and noninvasive method for the diagnosis of acute rejection after liver transplantation.Methods The allogeneic, syngeneic and immunosuppresive models of orthotopic rat liver transplantation were established, and acute rejection was graded according to histopathological change. Bile was sent for cytology by microscopy,and the sequential detection of bile IL 2,IFN ?,IL 4 and IL 6 gene expression was performed at day 1 through day 7. Results The number of cells in bile was persistently high in allogeneic group,and blast and lymphocytes appeared in bile. IL 2 and IFN ? were detected only in allogeneic group,with a specificity of 70% and 67%(7/10 and 8/12),and a sensitivity of 39% and 44%(7/18 and 8/18)respectively.Conclusion Bile cytology and detection of IL 2 and IFN ?mRNA transcript are useful adjuvant diagnosic methods for acute rejection after liver transplantation.
