Simultaneous Determination of the Contents of Fleroxacin and Tinidazole in Compound Fleroxacin Suppository by RP-HPLC
- VernacularTitle:RP-HPLC同时测定复方氟罗沙星栓中氟罗沙星和替硝唑的含量
- Author:
Limin GUO
;
Kewu XIAO
;
Zhiqiang REN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
compound fleroxacin suppository;
fleroxacin;
tinidazole
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the contents of fleroxacin and tinidazole in compound fleroxacin suppository METH_ODS:RP-HPLC was used,the detecting conditions:ODS-C18 column;mobile phase,0 05mol/L lemon acid-acetonitrile(65∶35),was modulated to pH=4 0 with triethylamine;flow rate 0 8ml/min;external standard method;detective wavelength 300nm RESULTS:Linear ranges were 9 92～79 36?g/ml(for fleroxacin) 20 32～162 56?g/ml(for tinidazole) respectively wh_ere the peak areas were correlative with the concentrations,r=0 9 974 and 0 9 995 The average recoveries were 101 6% and 100 1% and RSDs was 1 4% and 1 2% respectively CONCLUSION:This detecting method is simple,rapid,accurate and suitable for determination of the contents of this preparation
