Apoptosis of Cochlear Cells in Guinea Pigs With Kanamycin Ototoxicity
- VernacularTitle:卡那霉素耳中毒后豚鼠耳蜗细胞凋亡的研究
- Author:
Zezhang TAO
;
Jianfeng LIU
;
Bokui XIAO
- Publication Type:Journal Article
- Keywords:
Kanamycin Ototoxicity Apoptosis
- From:
Journal of Audiology and Speech Pathology
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether cochlear hair cells occur apoptosis following kanamycin treatment in guinea pigs. Methods It was comprised of two groups of six animals each. Experiment group and control group were subjected to administration of kanamycin,im,400 mg?kg -1 ?d -1 ,and saline respectively for successive 10 days. One week later, animals were sacrificed. Left cochlea and right cochlea were taken for cochlea preparation and cochlea section respectively. Apoptosis was detected by terminal deoxynucleotidyl transferase(TdT)-mediated deoxyuridine triphosphate(d-UTP) nick end-labling(TUNEL) method.Results Apoptosis was present in cochlear hair cells, supporting cells as well as marginal cells of stria vascularis except for the spiral ganglion cells in kanamycin treatment guinea pigs. No apoptosis was detected in control animals. Conclusion Apoptosis of cochlear cells play important role in kanamycin ototoxicity.
