Effects of low and high shear stress on tight junctions of endothelial cells
- VernacularTitle:高、低剪切力对内皮细胞紧密连接的影响
- Author:
Qiang SHU
;
Xin JIN
;
Guangli LING
- Publication Type:Journal Article
- Keywords:
Endothelium;
Permeability;
Hemodynamics
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of high and low shear stress on endothelial permeability and tight junctions(TJs). METHODS: Rabbit abdominal aortas in normal and high fat diet groups were stenosed to 60.7%?7.0% of original cross areas, they were sacrificed after 7 days, and the arteries were stained with Evans blue and Sudan Ⅳ. TJs were investigated with freeze fracture. RESULTS: The non-stenosis aortas were negative, there are 1 mm-wide constant and circular positive areas proximal and distal to the stenosis and scattered, dotted and patched positive areas within 8 mm distal to stenosis. Freeze fracture reveals that in the hyperpermeable regions, the percentages of zonular type of TJs and the numbers of strands of TJs of zonular and macular types in the distal regions are significantly lower than those in the proximal regions (P
