Study on polymorphism of human eighth complement component(C8A)by using allele specific amplification method
- VernacularTitle:用等位基因特异扩增技术检测人类补体第八成份C8A DNA多态性的研究
- Author:
Lin ZHANG
;
Junping XIN
;
Ronghua LI
- Publication Type:Journal Article
- Keywords:
Human eighth complement component;
Complement polymorphism;
Allele specific amplification
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
For the purpose of detecting the polymorphism of the human eighth complement component(C8A),an allele specific amplification method were established for detecting two alleles of C8A based on a nucleotide exchange of the C8? cDNA sequence.100 DNA blood samples from unrelated individuals of Han population in Chengdu were typed by this method.The result of comparison with the data of protein typing of the same samples using SDS PAGE method proved that the described method is efficient,correct and reliable for C8A genotypes.This methtod is valuable for further application in population genetic study and forensic science practice.
