Determination of the Content of Lomustine in Mouse Tumor Tissue by RP-HPLC
- VernacularTitle:RP-HPLC测定小鼠肿瘤组织中洛莫司汀的含量
- Author:
Yong SUN
;
Lixing ZHANG
- Publication Type:Journal Article
- Keywords:
lomustine;
RP - HPLC;
tumor tissue
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determine the content of lomustine in mouse tumor tissue. METHODS: Lomustine was separated and determined on a reverse-phase C18 5um column with a mobile phase of acetonitrile-H2O(54 : 46), detected at 254nm and no internal standard. RESULTS: The ca[ibration curve was 1ineaI(r = 0. 9 999) within the range of 0. 98- 15. 68ug/ml for lomus tine. The recovery ratio of lomustine was 68. 64% (n = 9). The relative standard deviation(RSD) was 0. 32%. CONCLUSION: This method is simple and accurate
