The protection of aminoguanidine,silymarin and anisodamine on growth and cytosolic free calcium changes of retinal capillary pericytes cultured in glycation products
- VernacularTitle:氨基胍、水飞蓟素、山莨菪碱对糖基化产物培养下周细胞增殖和胞浆钙改变的保护作用
- Author:
Xinghua XI
;
Deyong JIANG
;
Luosheng TANG
- Publication Type:Journal Article
- Keywords:
Pericytes/drug effects;
Calcium/analysis;
Silymarin;
Anisodamine;
Guanidines
- From:
Chinese Journal of Ocular Fundus Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of aminoguanidine(AG),silymarin (Sil) and anisodamine (Ani) on retinal capillary pericytes cultured in glycosylation products. Methods MTT cololrimetric assay, thymidine incorporating and fluorescent indicator fura 2 acetoxy methyl ester (Fura 2AM) were used to study the influence of AG,Sil and Ani on the growth,DNA synthesis,and cytosolic free calcium([Ca 2+ ]i)changes of pericytes cultured in the medium contained early glycation products (EGs) or advanced glycation end products (AGEs). Results Cultured in the medium contained EGs,the A value by MTT assayed and amount of thymidine incorporating in AG group and Sil group were obviously elevated than those of control group(P
