Matrix metalloproteinase in human diabetic and non-diabetic vitreous
- VernacularTitle:糖尿病及非糖尿病视网膜病变患者玻璃体中基质金属蛋白酶的表达
- Author:
Ming JIN
;
Kenji KASHIWAGI
;
Shigeo TSUKAHALA
- Publication Type:Journal Article
- Keywords:
Diabetic retinopathy;
Retinal detachment;
Matrix metalloproteinases/analysis;
Retina
- From:
Chinese Journal of Ocular Fundus Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To identify matrix metalloproteinase (MMP) in human vitreous samples of diabetic vitreoretinopathy (DR) and other ocular diseases (non DR) and to probe the related factors of MMP expression. Methods Thirty one diabetic and 17 non diabetic vitreous samples (nine macular hole and eight epiretinal membrane patients) were examined. Samples were concentrated and subjected to substrate zymography to conduct a quantitative analysis of MMP 2,9 activity. The technology of Western blotting against anti human MMP 2,9 was performed to identify MMP in vitreous samples. Results Vitreous samples both from DR patients and from non DR patients showed a single band at the position of 72 kDa, corresponding to MMP 2. Quantitative analysis revealed that diabetic vitreous showed higher MMP 2 activity than non DR, although the difference was not significant. 45.2% of DR patients showed MMP 9, but no expression in non DR. Among DR samples, the positive ratio of MMP 9 in partial posterior vitreous detachment (PVD)(66.7%) was significantly higher than that of complete PVD (15.4%). Western blotting study confirmed the expression of MMP 2 and MMP 9. Conclusion There is no obvious difference of MMP 2 activity between DR and non DR. MMP 9 may be involved in the pathogenesis of diabetic vitreoretinopathy and the deterioration of proliferative change.
