Experimental study on targeting transfer of reporter gene in proliferative vitreoretinopathy of rabbit
- VernacularTitle:增生性玻璃体视网膜病变模型眼内定向转染报告基因的研究
- Author:
Guangming WAN
;
Shuiqing ZENG
- Publication Type:Journal Article
- Keywords:
Proliferative vitreoretinopathy/therapy;
Gene therapy;
Reporter gene
- From:
Chinese Journal of Ocular Fundus Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To study the possibility of prevention of proliferative vitreoretinopathy(PVR) by transduction of exogenous gene in vivo. Methods PVR model of rabbits was induced by intravitreal injection of fibroblasts.? galactosidase (lacZ) gene as a reporter gene was transfered into the vitreous of PVR model eyes mediated by retroviral vector, and the expression of the gene in eye tissues was determined. Gene transfection was done on the 6th day after fibroblasts injection,and the dosage of intravitreal injection of reporter gene was 0.1ml PLXSN/lacZ serum free supernatant (1.1?10 6 cfu/ml). Results lacZ gene expression was seen in proliferative membranes after gene transfection, and the expression was located maily at the surface of PVR membrane. The reporter gene expression lasted at least more than 30 days. No expression was found in retinal tissues. Conclusions Retrovirus mediated gene can be directionally transducted in PVR membrane,and might possess the feasibility of gene therapy for PVR. [
