Effect of transfection of tumor necrosis factor ? gene and its mutants on tumorigenicity of H22 tumor cells in vivo
- VernacularTitle:转染肿瘤坏死因子?及其突变体基因对H22肿瘤细胞体内致瘤能力的影响
- Author:
Qingfen LI
;
Zhuoya LI
;
Feili GONG
;
Yong XU
;
Xiaodan JIANG
;
Wei FENG
;
Ping XIONG
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor;
Gene expression;
Lymphocytes;
Neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To compare the tumorigenecity of H22 cells transfected with TNF-? gene and its mutants (secreted TNF-? mutant, S-TNFm, transmembrane TNF-? mutant, TM-TNFm and wild type of TNF-?, Wt-TNF) in vivo . METHODS: Three kinds of mouse liver cancer cell line H22 expressing TNF-? and its two mutants were mixed with untransfected H22 at different effector/target ratio separately. The growth of tumor was examined after injection of 2.5?10 5 (100 ?L) mixed H22 tumor cells into mice. The lymphocyte infiltration in the site of tumor and the expression of Fas on tumor cells were detected by immunohistochemistry. RESULTS: The tumorigenecity of H22 cells transfected with TNF-? gene and its mutants was significantly weakened ( P
