Deletion of the LMP-1 gene integrated in the nasopharyngeal carcinoma cell line SUNE-1
- VernacularTitle:整合在鼻咽癌细胞株SUNE-1中的EB病毒LMP-1基因的缺失性改变
- Author:
Peng LIU
;
Zhenyu ZHU
;
Qingxiu ZHANG
;
Bijun HUANG
;
Manzhi LI
;
Danyun HUANG
;
Jianqua MA
- Publication Type:Journal Article
- Keywords:
Epstein-Barr virus;
Gene deletion;
Nasopharyngeal neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the EBV LMP-1 gene integrated in the chromosome of poorly differentiated nasopharyngeal carcinoma cell line SUNE-1. METHODS: The LMP-1 gene of SUNE-1 was detected with PCR; Deletion of LMP-1 was examined by restriction endonuclease analysis and PCR. The deletion was precisely localized by DNA sequencing. RESULTS: The LMP-1 gene integrated in the chromosome of SUNE-1 could be deleted or non-deleted. The two introns of LMP-1 gene were shown being lost in SUNE-1 cell line. CONCLUSION: Deletion of intron 1 and intron 2 happen in some of the LMP-1 gene integrated in the chromosome of SUNE-1.
