Interleukin-6 induced upregulation of basic fibroblast growth factor isoform through phorbol ester-sensitive protein kinase C in rat vascular smooth muscle cells
- VernacularTitle:佛波酯敏感的PKC介导IL-6上调大鼠血管平滑肌细胞bFGF蛋白表达
- Author:
Xiaohong PAN
;
Jiang SHAN
;
Jianhon LUO
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor,basic;
Interleukin-6;
Protein kinase C;
Muscle,smooth,vascular
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The role of protein kinase C(PKC) in the effect of Interleukin-6(IL-6) on basic fibroblast growth factor(bFGF) expression was investigated in rat vascular smooth muscle cells(VSMC). METHODS: Western-blotting was adopted to observe the variation of bFGF and its receptor type I isoforms expression. RESULTS: IL-6 increased all the three basic fibroblast growth factor isoforms in a dose-dependent (0-10.0 ?g/L) manner. The upregulatory activities peaked at 24 h as demonstrated . In addition, after exhaustion of intracellular phorbol ester-sensitive PKC, the upregulatory effects of IL-6 on bFGF exprssion in VSMC declined ( P
