Inhibitory effect of canstatin RNA transfection on the growth of cultured rabbit vascular smooth muscle cells
- VernacularTitle:Canstatin RNA转染对兔血管平滑肌细胞体外增殖的抑制作用
- Author:
Lang LI
;
Jianzhang FENG
;
Wenling ZHENG
;
Jiang XIAN
;
Hongbin ZHANG
;
Taicheng YANG
;
Qidong TANG
;
Chuny DENG
- Publication Type:Journal Article
- Keywords:
Gene transfer;
Muscle, smooth,vascular;
RNA
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of canstatin on cultured rabbit vascular smooth muscle cells(VSMC). METHODS: By means of cationic liposome mediated method, canstatin RNA was transferred into cultured VSMC. The proliferation quantity of VSMC were determined by the cell counting method and thymidine(-TdR) incorporation. RESULTS: Canstatin RNA could be effectively transferred into cultured primary rabbit aortic smooth muscle cells by the cationic liposome-Dosper and could markedly inhibit VSMC proliferation. CONCLUSION: Transfection of canstatin RNA could inhibit the growth of VSMC in vitro.
