The cloning of a new liver cancer related gene and preparation of the antibody
- VernacularTitle:一个富含脯氨酸肝癌新基因的克隆和抗体制备
- Author:
Zhengxu WANG
;
Mengchao WU
;
Hongyang WANG
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Cloning, molecular;
Oncogene proteinssion;
Expression
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone a new liver cancer associated gene, and to explore the molecular basis of liver cancer genesis. Methods Using mRNA differential display polymerase chain reaction (DDPCR), we investigated the difference in mRNA expression between primary hepatocellular carcinoma and paracarcinoma liver tissue, and achieved a gene probe. The target gene expression was proved in parent tissue by Northern blot. Screening the placenta cDNA library, we got a full length cDNA of this gene. By GST fusion protein methods, we prepared the special polyantibody of C end of the gene. We identified the polyantibody and the expression of this gene by Western blot method. Results We obtained a full length cDNA of proline riched cancer associated gene, and we have prepared the specific polyantibody of this gene. Conclusion The obtaining of a novel liver cancer associated gene and successful preparation of the polyantibody pave the way for further study on gene therapy for liver cancer.
