Effect of Taurine on the Cisplatin-induced Changes of Membrane Mobility of Primary Cultured Renal Proximal Tubular Cells in Rabbits
- VernacularTitle:牛磺酸对顺铂引起的原代培养兔肾小管上皮细胞膜流动性的影响
- Author:
Shiwen ZHOU
;
Shijie LIU
- Publication Type:Journal Article
- Keywords:
cisplatin;
taurine;
proximal kidney tubular cell;
membrane mobility
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the changes of membrane mobility of primary cultured renal proximal tubular cells(PTC) induced by cisplatin and to explore the protective effect of taurine on the changes.METHODS:PTC were established in vitro.Cisplat_in-induced groups:PTC were incubated with cisplatin(6.5,13,26,52?mol/L) for 24h.Taurine groups:PTC were preincubated with taurine(0.1,1,10g/L) for 24h.Then cisplatin(26?mol/L) was added into the culture and continued to incubate for 24h.The membrane mobility of PTC was measured using DPH(1,6-Diphenyl-1,3,5 hexatriene)as fluorescence probe.RESU_LTS:Cisplatin(13,26,52?mol/L) significantly increased the membrane mobility of PTC.The degree of fluorescence polarization(P),the anisotropy(?) and the microviscosity(?) of PTC were significantly reduced(P
