Determination of Prodatin in Polygonum Cuspidatum Injection With HPLC
- VernacularTitle:高效液相色谱法测定虎杖注射液中白藜芦醇苷含量
- Author:
Jianhai LIN
;
Yuenian TANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Polygonum cuspidatum;
prodatin
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for determination of prodatin in Polygonum cuspidatum injection.M_ETHODS:The analytical column was Hypersil ODS column(100mm?2.1mm,5?m).The mobile phase consisted of methanol-water(25∶75).The flow rate was 1.0ml/min.The UV detection wavelength was 290nm.RESULTS:The linear range was 0.145～2.32?g/ml(r=0.9 999).The average recovery of prodatin was 98.8%(RSD=1.15%).CONCLUSION:The method is convenient,accurate and could be used for quality control in the production of Polygonum cuspidatum injection.
