The Imitative experiment on gastroscopic disinfection── determination of cleaning and disinfectant time
- VernacularTitle:胃镜消毒模拟试验──清洗与消毒时间探讨
- Author:
Yanfang GONG
;
Guoming XU
;
Yuqiang FANG
- Publication Type:Journal Article
- Keywords:
Gastroscope;
Glutaraldehyde;
Disinfection;
Imitative experiment
- From:
Chinese Journal of Digestive Endoscopy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the cleaning and the shortest disinfectant time for gastroscope Methods 1? g/ml HBsAg and 3? 108/ml bacteria of Aureus Staphylococci(ATCC 6538),Escherichia coli(ATTC 8099),Bacterium earuginosum(ATCC 27853)were artificially spreaded on the body of gastroscope ,biopsy hole and biopsy clamp.The gastroscope was routinely cleaned and disinfected.HBsAg and pathogens in the samples,which collected before and after cleaning the gastroscope and 3min,5min,10min after disinfection,were tested by using ELISA and bacteria culture,respectively.Results Pathogen and HBsAg can be detected before cleaning the gastroscope.Pathogen can also be detected,but HBsAg disappears after cleaning.Both HBsAg and pathogen was negative 3 min after gastroscope was immersed in glutaraldehyde.Conclusion The tests for pathogens and HBsAg can be negative after gastroscope was cleaned and immersed in 2％ glutaraldehyde for 3 min.
