Effect of isoflurane on lung ischemia-reperfusion injury in rabbit
- VernacularTitle:异氟醚对家兔肺缺血-再灌注损伤的影响
- Author:
Yanping CHEN
;
Dequan CAO
;
Yetian CHANG
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Lung;
Reperfusion injury
- From:
Chinese Journal of Anesthesiology
1996;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of isoflurane on lung ischemia reperfusion injury in rabbit in vivo Methods Thirty two healthy New Zealand white rabbits of either sex weighing 2 0 2 5kg were randomly divided into four groups of eight each:sham operation (group A): chest was opened and left main bronchus and pulmonary artery and vein were isolated but not clamped The lungs were ventilated for 120min; ischemia reperfusion (group B): left hilum was isolated and clamped for 60min, after declamping the lungs were ventilated for another 60min Isoflurane+ischemia reperfusion(group C): the lungs were first ventilated with 1MAC isoflurane for 15min then the left hilum was clamped for 60min, after declamping the lungs were ventilated with 1MAC isoflurane for another 60min Isoflurane(group D): left hilum isolated but not clamped The lungs were ventilated with 1MAC isoflurane for another 60min Animals were then killed by blood letting, left lung was excised for microscopic examination and measurement of wet/dry weight(W/D) ratio and MDA content Results The W/D ratio and MDA content of left lung were significantly higher in group B and group C than those in group A and group D, while W/D ratio and MAD content in group B were significantly higher than those in group C Microscopic examination showed that there were severe leukocyte infiltration and edema formation in alveolar spaces and alveolar structure was destroyed in group B and group C, but the changes were less severe in group C Conclusions Inhalation of 1MAC isoflurane before ischemia and during reperfusion protects the lungs against ischemia reperfusion injury in rabbit in vivo
