Role of cyclic nucleotides in the acute hypoxic responses of hypoxic subcultured porcine pulmonary arterial smooth muscle and endothelial cells
- VernacularTitle:连代低氧培养肺动脉平滑肌及内皮细胞的急性低氧反应中环核苷酸的作用
- Author:
Qing ZENG
;
Xianrong JIN
;
Dixun WANG
;
Zhigang HONG
;
Tianling HAO
;
Zongbin XIONG
;
Bingyon SUN
- Publication Type:Journal Article
- Keywords:
Nucleotides, cyclic;
Cells;
Anoxia;
Pulmonary artery;
Endothelium;
Muscle,smooth
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect the role of cyclic nucleotides in the alleviation of hypoxic pulmonary vasoconstriction (HPV) in chronic hypoxic animals. METHODS: The intracellular cAMP and cGMP of the cultured porcine pulmonary arterial smooth muscle cells (PASMC) and endothelial cells (PAEC) were assayed by RIA. The length of single PASMC during acute hypoxia was measured by imaging analysis system. RESULTS: The basal levels of cAMP and cGMP in PASMC and cGMP in PAEC of Chronic hypoxic groups decreased remarkably compared with normoxic groups ( P
