The antisense phosphorothioate oligodeoxynucleotides of bcl-2 oncogene enhances the apoptotic effect of As_2O_3 on NB4 leukemic cells
- VernacularTitle:Bcl-2基因反义核酸增强三氧化二砷对NB4细胞凋亡效应
- Author:
Weili SHEN
;
Yuan ZHANG
- Publication Type:Journal Article
- Keywords:
Oligonucleotides, antisense;
Genes, bcl-2;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study and explore whether the antisense phosphorothioate oligodeoxynucleotides (ASODN) of bcl-2 oncogene would increase the apoptotic effect of As 2O 3 on NB4 leukemic cells. METHODS: The biological and morphological changes in NB4 cells from microculture with As 2O 3, bcl-2 ASODN or both were observed. The changes in DNA content and Bcl-2 protein of NB4 cells from microculture with As 2O 3, bcl-2 ASODN or both were determined by tissue chemistry and flowcytometry. RESULTS: There was much more apoptotic effect of As 2O 3 on NB4 cells while it combined with bcl-2 ASODN (ASODN 10 0 ?mol/L+ As 2O 3 0 25 ?mol/L) than alone(ASODN 10 0 ?mol/L or As 2O 3 0 25?mol/L),and so did inhibitory effect of Bcl-2 protein expression by flow cytometry. CONCLUSION: The bcl-2 ASODN can enhance the apoptotic effect of As 2O 3 on NB4 leukemic cells. [
