A method of inducing mouse embryonic stem cells cultured in vitro to differentiate into cardiomyocytes
- VernacularTitle:诱导体外培养小鼠胚胎干细胞分化为心肌细胞方法的研究
- Author:
Qiutang ZENG
;
Linsheng CAO
;
Wuqiang ZHU
- Publication Type:Journal Article
- Keywords:
Embryo;
Stem cells;
Myocardium;
Cells
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the method of inducing mouse embryonic stem (ES) cells in vitro to differentiate into cardiomyocytes without using any chemical reagents.METHODS: BRL conditioned medium was used to promote the growth of ES cells and maintain them in an undifferentiated state before the experiment of differentiation. Then a three-step method including ES cell culture in hanging drops and in suspending was used to induce the differentiation of ES cells. RESULTS: Rhythmically contracting cells were observed among differentiated cells, which were proved to be cardiomyocytes with electron microscope and immunocytochemistry. CONCLUSION: A simple and economical method was established to induce mouse ES cells cultured in vitro to differentiate into cardiomyocytes without using any chemical reagents. [
